Open AccessZnSe:Mn/ZnS quantum dots for the detection of microcystin by room temperature phosphorescence immunoassay
Author(s) -
You Jiaqi,
Ma Long,
He Yu,
Ge Yili,
Song Gongwu,
Zhou Jiangang
Publication year - 2019
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2018.5690
Subject(s) - phosphorescence , quantum dot , immunoassay , detection limit , carbodiimide , analytical chemistry (journal) , chemistry , luminescence , linear range , materials science , fluorescence , nanotechnology , optoelectronics , chromatography , optics , organic chemistry , antibody , physics , immunology , biology
This work has assembled ZnSe:Mn/ZnS quantum dots (QDs)‐monoclonal anti‐microcystin‐Leucine Arginine (anti‐MC‐LR) antibody bioconjugates as a room temperature phosphorescence (RTP) probe using 1‐ethyl‐3‐(3‐dimethyllaminopropyl) carbodiimide/N‐Hydroxysuccinimide chemistry for detecting MC‐LR. Once the specific interactions occurred between these MCs and their antibodies on the surface of ZnSe:Mn/ZnS QDs, the RTP of ZnSe:Mn/ZnS QDs was quenched with the increased concentration of MC‐LR. Under the optimised conditions, the linear range of MC‐LR was determined to be 0.9–8.8 nM. The linear relationship between ( P 0 – P )/ P 0 and concentration of MC‐LR were observed with the regression equation ( P 0 – P )/ P 0 = 0.03908C + 0.0456 with the detection limit of 0.038 nM. The developed RTP immunoassay favoured environmental applications since the interference from autofluorescence and scattering light was greatly eliminated.