Open AccessLabel‐free immunosensor based on interdigitated electrodes with Ni–Co nanowires
Author(s) -
Siao FuYuan,
Chang YawJen,
Shih HungJu
Publication year - 2018
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2018.5135
Subject(s) - analyte , materials science , electrode , nanowire , nanotechnology , anode , potentiometric titration , analytical chemistry (journal) , chromatography , chemistry
This work presents a label‐free immunosensor consisting of interdigitated electrodes and Ni–Co nanowires (NWs) to determine the relative amount of analyte, using electroanalytical measurement. Interdigitated electrodes were fabricated by the conventional printed circuit board treatment and Ni–Co NWs were synthesised by the electrodeposition method using a porous anodic alumina oxide membrane as the template. The experimental results showed that Ni–Co NWs can provide specific binding with histidine‐tagged capture agent based on the method of immobilised metal affinity chromatography. Due to the large surface‐to‐volume ratio of NWs, this immunosensor had 2.6‐fold the immobilisation ability of flat Ni–Co film to absorb histidine‐tagged capture agent. Moreover, this immunosensor exhibited sensitivity in detecting low amounts of analyte. The electrical responses of the electroanalytical measurement of analyte, from 1000 to 625 ng/mL, could be discriminated; however, the fluorescence intensities became indiscriminative. This label‐free immunosensor has the potential to immobilise various capture agents through the use of histidine for different analyte analyses.