Open AccessMapping of accumulation of SeNPs in developing zebrafish embryos and larvae: a new method using SEM with energy dispersive X‐ray spectrometer
Author(s) -
Kalishwaralal Kalimuthu,
Jeyabharathi Subhaschandrabose,
Sundar Krishnan,
Muthukumaran Azhaguchamy
Publication year - 2017
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2016.0692
Subject(s) - blastula , zebrafish , embryo , scanning electron microscope , larva , biology , embryogenesis , materials science , microbiology and biotechnology , botany , biochemistry , gastrulation , composite material , gene
Zebrafish embryos have been used as a popular and ideal model system for the study of drug delivery and nanotoxicology. In the present study, synthesised selenium nanoparticles (SeNPs) were characterised using scanning electron microscope (SEM) with energy dispersive X‐ray (EDX) spectrometer. Early blastula stage (48 h post‐fertilisation) zebrafish embryos and larvae were treated with SeNPs, and SeNPs exposed embryos and larvae were fixed with 1% of formaldehyde and analysed for the accumulation of SeNPs in zebrafish. Here, a new method for studying SeNPs accumulation and surface distribution in zebrafish embryos and larvae using SEM‐EDX mapping is reported.