Open AccessSimultaneous determination of opioid drugs in urine with high‐performance liquid chromatography–ultraviolet after supramolecular based magnetic NP solid‐phase extraction
Author(s) -
Zabardasti Abedien,
Afrouzi Hossein,
Kakanejadifard Ali,
AmoliDiva Mitra
Publication year - 2017
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2016.0603
Subject(s) - chromatography , solid phase extraction , extraction (chemistry) , chemistry , detection limit , codeine , high performance liquid chromatography , sodium dodecyl sulfate , elution , morphine , pharmacology , medicine
A simple and rapid extraction/preconcentration procedure using supramolecular based magnetic nanoparticle (MNP) solid‐phase extraction method by sodium dodecyl sulphate (SDS)‐coated MNPs has been developed for both removal and preconcentration of three important opioid drugs named morphine, codeine and methadone before their high‐performance liquid chromatography–ultraviolet determination. To get insight conguration of SDS molecules on the surface of the MNPs, zeta potential measurements were performed in different SDS (mg)/mg of MNPs ratios. Parameters affecting the extraction and preconcentration efficiency of the opioids were optimised as recovery by one variable at‐a‐time methodology and the obtained recoveries were 91, 94 and 98% for morphine, codeine and methadone, respectively. Under optimum conditions (such as pH = 3.5; adsorbent amount = 60 mg; eluent solvent volume = 2 ml of acetonitrile, sample volume = 200 ml etc.), the method was linear in the range of 1–200 ng ml −1 with enrichment factors (EFs) of 91, 94 and 98 for morphine, codeine and methadone, respectively, and the limits of detection were <0.27 ng ml −1 . Finally, the proposed method was successfully applied for both removal and trace determination of the targeted drugs in urine samples.