Open AccessElectrodeposited nanoscale zinc oxide particles: facilitating the electron transfer of immobilised protein and biosensing
Author(s) -
Bayandori Moghaddam Abdolmajid
Publication year - 2017
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2016.0563
Subject(s) - biosensor , electron transfer , electrochemistry , chemistry , electrode , redox , hydrogen peroxide , platinum , zinc , molecule , inorganic chemistry , nanoparticle , materials science , nanotechnology , photochemistry , organic chemistry , catalysis
The electron transfer of redox‐active sites of immobilised haemoglobin (Hb) has been studied by voltammetric experiments. The electrode surface was modified by nanoparticles (NPs) to make a suitable substrate for electric wiring of protein molecules and efficient electron transferring. Hence, the Hb/zinc oxide NPs/platinum electrode was prepared by chronoamperometric deposition of the NPs and immobilisation of protein molecules. The electrochemical behaviour of the prepared biosensor was quasi‐reversible and showed a pair of redox peaks with a formal potential ( E 0′ ) of 214 mV. Additionally, the electron transfer rate constant ( k s ) of immobilised Hb was about 0.621 s −1 . Biocatalytic reduction of hydrogen peroxide was also examined on the prepared biosensor. Result indicated to good sensitivity, wide linear range and low detection limit of 6 μM.