Open AccessDetermination of hydrogen peroxide using a biosensor based on Fe 3 O 4 magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite
Author(s) -
Qu Jianying,
Dong Ying,
Wang Yong,
Lou Tongfang,
Du Xueping
Publication year - 2014
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2014.0352
Subject(s) - biosensor , horseradish peroxidase , graphene , hydrogen peroxide , dielectric spectroscopy , fourier transform infrared spectroscopy , detection limit , cyclic voltammetry , materials science , nuclear chemistry , nanoparticle , analytical chemistry (journal) , chitosan , chemistry , chemical engineering , electrochemistry , nanotechnology , chromatography , electrode , organic chemistry , engineering , enzyme
A novel hydrogen peroxide (H 2 O 2 ) biosensor was constructed based on Fe 3 O 4 magnetic nanoparticles (MNPs) and horseradish peroxidase with graphene–chitosan composite material as a matrix. Fe 3 O 4 MNPs were synthesised by chemical co‐precipitation with sodium citrate as surfactant. Fourier transform infrared spectroscopy and transmission electron microscopy were applied for the characterisation of the obtained Fe 3 O 4 MNPs. The conductivity of different composite films was researched by electrochemical impedance spectroscopy and the electrocatalytic properties of the H 2 O 2 biosensor were studied by cyclic voltammetry. Under optimal conditions, experimental results indicated that the biosensor could electrocatalyse the reduction of H 2 O 2 ; the reduction peak current had a good linear relationship with the concentration of H 2 O 2 from 2.49 × 10 −5 to 1.67 × 10 −3 mol/l ( R = 0.9990). The detection limit was 3.05 × 10 −6 mol/l ( S/N = 3). This novel biosensor showed good sensitivity, stability and repeatability for H 2 O 2 detection.