Determination of hydrogen peroxide using a biosensor based on Fe 3 O 4  magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite | Zendy

Qu Jianying | Zendy; Dong Ying | Zendy; Wang Yong | Zendy; Lou Tongfang | Zendy; Du Xueping | Zendy

Open Access

Determination of hydrogen peroxide using a biosensor based on Fe 3 O 4 magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite

Author(s) -

Qu Jianying,

Dong Ying,

Wang Yong,

Lou Tongfang,

Du Xueping

Publication year - 2014

Publication title -

micro and nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.25

H-Index - 31

ISSN - 1750-0443

DOI - 10.1049/mnl.2014.0352

Subject(s) - horseradish peroxidase , hydrogen peroxide , graphene , biosensor , nanoparticle , chitosan , peroxidase , composite number , magnetic nanoparticles , nanotechnology , materials science , chemistry , nuclear chemistry , chemical engineering , inorganic chemistry , organic chemistry , enzyme , composite material , engineering

A novel hydrogen peroxide (H 2 O 2 ) biosensor was constructed based on Fe 3 O 4 magnetic nanoparticles (MNPs) and horseradish peroxidase with graphene–chitosan composite material as a matrix. Fe 3 O 4 MNPs were synthesised by chemical co‐precipitation with sodium citrate as surfactant. Fourier transform infrared spectroscopy and transmission electron microscopy were applied for the characterisation of the obtained Fe 3 O 4 MNPs. The conductivity of different composite films was researched by electrochemical impedance spectroscopy and the electrocatalytic properties of the H 2 O 2 biosensor were studied by cyclic voltammetry. Under optimal conditions, experimental results indicated that the biosensor could electrocatalyse the reduction of H 2 O 2 ; the reduction peak current had a good linear relationship with the concentration of H 2 O 2 from 2.49 × 10 −5 to 1.67 × 10 −3 mol/l ( R = 0.9990). The detection limit was 3.05 × 10 −6 mol/l ( S/N = 3). This novel biosensor showed good sensitivity, stability and repeatability for H 2 O 2 detection.

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