Open AccessManipulation of DNA origami nanotubes in liquid using programmable tapping‐mode atomic force microscopy
Author(s) -
Li Longhai,
Tian Xiaojun,
Dong Zaili,
Liu Lianqing,
Tabata Osamu,
Li Wen J.
Publication year - 2013
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2013.0483
Subject(s) - atomic force microscopy , tapping , materials science , nanotechnology , nanoscopic scale , mica , substrate (aquarium) , conductive atomic force microscopy , vibration , composite material , acoustics , physics , oceanography , geology
The nanoscale manipulation of 1D soft and flexible ‘DNA origami nanotubes’ (DONs) that are 6 nm in diameter and 400 nm in length, and placed on a mica substrate in a TE/Mg 2+ buffer solution, was executed by an atomic force microscopy (AFM) tip using a programmable tapping‐mode manipulation strategy. The AFM's tip tapping amplitude was controlled to be less than or equal to 10 nm while vibrating in a solution with Mg 2+ concentration of 10–20 mM. A series of single‐point and one‐step manipulation experiments revealed that manipulation can be achieved with an 80% successful rate under the condition that the AFM tip vibration amplitude is 3–4 nm and the Mg 2+ concentration is 10 mM. Utilising this optimised condition, multipoint and multistep manipulation based on the programmable tapping‐mode AFM process was conducted and nanomanipulation of DONs was successfully demonstrated.