Open AccessSynthesis of antibacterially bioinorganic composite by immobilising lysozymes in layered titanates
Author(s) -
Fu Jie,
Han Zhuping,
Ye Peng,
Dong Xiaoping
Publication year - 2013
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2013.0004
Subject(s) - intercalation (chemistry) , composite number , exfoliation joint , thermal stability , bioinorganic chemistry , chemistry , chemical engineering , enzyme , materials science , inorganic chemistry , composite material , organic chemistry , graphene , nanotechnology , engineering
An antibacterially bioinorganic composite has been synthesised by intercalating lysozymes (LZs) into layered titanates with a convenient and efficient exfoliation‐restacking strategy. This composite exhibits a loose slab morphology with a thickness of 5–10 layers, and the interlayer space is ∼4.4 nm because of intercalation of enzymes. The immobilised amount of lysozymes is up to ∼68.3% in weight because of the layer‐by‐layer alternate and swollen structure. This composite is stable in the neutral and weakly acidic condition, and only releases <10% of lysozymes in the pH > 4 solution. The immobilised LZs exhibit excellent thermal stability, which retain their initial activities of about 70% at 70°C for about 40 min. In addition, the residual activities of the immobilised enzymes are 68% after ten recycles reuse.