Open AccessFlow‐lysometry for cytoplasmic analysis of single cells in large populations
Author(s) -
Lee Won Chul,
Pisano Albert P.,
Cho YoungHo,
Kuypers Frans A.
Publication year - 2013
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2012.0722
Subject(s) - flow (mathematics) , cytoplasm , biology , microbiology and biotechnology , mechanics , physics
Presented is a single‐cell analyser to measure cytoplasmic components in large cell populations. This technology, flow‐lysometry, uses a flow‐cell that performs three functions (cell detection, cell lysis and component sensing) in a continuous microfluidic channel. This flow‐cell can be coupled and synchronised with classical flowcytometry, thus offering high‐throughput measurements of various cytoplasmic compounds in combination with cell‐surface markers. The measurement of cytoplasmic markers in the cytosol of red blood cells was shown to verify population analysis of individual cells in mixed‐cell populations. This work presents an approach to characterise complex cell populations to extend measurements in cell biology.