A novel Fe 3 O 4  magnetic nanoparticles functionalized by glutamic acid and conjugated with thiosemicarbazide alter the expression of  norB  gene, in  Staphylococcus aureus | Zendy

Safarnejad Erfan | Zendy; MoradiShoeili Zeinab | Zendy; Rahmatollahi Hamid Reza | Zendy; Jalali Amir | Zendy; Salehzadeh Ali | Zendy

Open Access

A novel Fe 3 O 4 magnetic nanoparticles functionalized by glutamic acid and conjugated with thiosemicarbazide alter the expression of norB gene, in Staphylococcus aureus

Author(s) -

Safarnejad Erfan,

MoradiShoeili Zeinab,

Rahmatollahi Hamid Reza,

Jalali Amir,

Salehzadeh Ali

Publication year - 2022

Publication title -

micro and nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.25

H-Index - 31

ISSN - 1750-0443

DOI - 10.1049/mna2.12109

Subject(s) - staphylococcus aureus , conjugated system , chemistry , nanoparticle , magnetic nanoparticles , microbiology and biotechnology , nuclear chemistry , bacteria , nanotechnology , materials science , biology , organic chemistry , polymer , genetics

Here, the improved efficacy of a novel composite of iron oxide (Fe 3 O 4 ) magnetic nanoparticles (MNPs) functionalized by glutamic acid (Glu) and conjugated with thiosemicarbazide (TSC), evaluated on the expression of norB efflux pump gene in S. aureus strains. The proper synthesis of Fe 3 O 4 @Glu–TSC NP was confirmed and characterized by Fourier transform infrared spectroscopy (FTIR), scanning and transmission electron microscopy, Brunauer–Emmett–Teller (BET), energy dispersive X‐Ray spectroscopy (EDX), X‐ray diffraction (XRD), and UV–vis spectroscopy. The effect of NP on the expression of norB gene was studied using quantitative reverse transcription polymerase chain reaction (qRT‐PCR). The physical analysis of NP revealed proper synthesis, dispersion, and stability, as well as the spherical/cubic morphology with a mean size of 35–60 nm. The minimum inhibitory concentration (MIC) value of the functionalized nanoparticle decreased by two folds towards each respected strain in contrast with the Fe 3 O 4 NP (alone). Additionally, in the presence of NP, the norB gene expression was reduced by up to 50% in comparison with the control group. In conclusion, the functionalized NP can be considered as a promising inhibitor of bacterial efflux pump genes against antibiotic‐resistant bacteria. However, further studies should be conducted to determine the safety of these nanoparticles for medicinal applications.

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