Open AccessFluorescence imaging under background light with a self‐reset complementary metal–oxide–semiconductor image sensor
Author(s) -
Yamaguchi Takahiro,
Sunaga Yoshinori,
Haruta Makito,
Motoyama Mayumi,
Ohta Yasumi,
Takehara Hiroaki,
Noda Toshihiko,
Sasagawa Kiyotaka,
Tokuda Takashi,
Ohta Jun
Publication year - 2015
Publication title -
the journal of engineering
Language(s) - English
Resource type - Journals
ISSN - 2051-3305
DOI - 10.1049/joe.2015.0046
Subject(s) - fluorescence , reset (finance) , pixel , image sensor , materials science , optoelectronics , semiconductor , fluorescent light , optics , green fluorescent protein , fluorescence lifetime imaging microscopy , chemistry , physics , biochemistry , financial economics , economics , gene
The authors propose and demonstrate the fluorescence imaging of green fluorescence protein (GFP) expressed in a brain slice with a self‐reset complementary metal–oxide–semiconductor image sensor under background light. By using a self‐reset function to avoid pixel saturation, the weak fluorescence of GFP was successfully observed, even under background light. The result suggests that the sensor can be applied to in vivo imaging of laboratory animals during light–dark cycles, so that they can observe the different responses to bright and dark environments.