Open AccessKinetic model of metabolic network for xiamenmycin biosynthetic optimisation
Author(s) -
Xu Minjuan,
Chen Yongcong,
Xu Jun,
Ao Ping,
Zhu Xiaomei
Publication year - 2016
Publication title -
iet systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.367
H-Index - 50
eISSN - 1751-8857
pISSN - 1751-8849
DOI - 10.1049/iet-syb.2014.0054
Subject(s) - glycerol , phosphoenolpyruvate carboxykinase , biochemistry , enzyme , flux (metallurgy) , streptomyces , synthetic biology , biology , chemistry , computational biology , biochemical engineering , biological system , bacteria , genetics , organic chemistry , engineering
Xiamenmycins, a series of prenylated benzopyran compounds with anti‐fibrotic bioactivities, were isolated from a mangrove‐derived Streptomyces xiamenensis . To fulfil the requirements of pharmaceutical investigations, a high production of xiamenmycin is needed. In this study,, the authors present a kinetic metabolic model to evaluate fluxes in an engineered Streptomyces lividans with xiamenmycin‐oriented genetic modification based on generic enzymatic rate equations and stability constraints. Lyapunov function was used for a viability optimisation. From their kinetic model, the flux distributions for the engineered S. lividans fed on glucose and glycerol as carbon sources were calculated. They found that if the bacterium can utilise glucose simultaneously with glycerol, xiamenmycin production can be enhanced by 40% theoretically, while maintaining the same growth rate. G lycerol may increase the flux for phosphoenolpyruvate synthesis without interfering citric acid cycle. They therefore believe this study demonstrates a possible new direction for bioengineering of S. lividans .