Open AccessBiodistribution of nanodiamonds in the body of mice using EPR spectrometry
Author(s) -
Inzhevatkin Evgeny,
Baron Alexey,
Maksimov Nikolai,
Volkova Marina,
Puzyr Alexey,
Ronzhin Nikita,
Bondar Vladimir
Publication year - 2019
Publication title -
iet science, measurement and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.418
H-Index - 49
eISSN - 1751-8830
pISSN - 1751-8822
DOI - 10.1049/iet-smt.2018.5594
Subject(s) - biodistribution , electron paramagnetic resonance , spleen , chemistry , in vivo , mass spectrometry , radiochemistry , in vitro , nuclear magnetic resonance , chromatography , medicine , biochemistry , biology , physics , microbiology and biotechnology
In vitro experiments proved the usefulness of electron paramagnetic resonance (EPR) spectrometry for detecting detonation nanodiamonds (NDs) in samples of biomaterials (blood and homogenates of organs of mice). A characteristic EPR signal ( g = 2.003, Δ H ≃ 10 G) was detected in biomaterials containing NDs, and its intensity linearly increased at nanoparticle concentrations of between 1.6 and 200 µg/ml. In vivo experiments demonstrated that EPR spectrometry was effective for monitoring the inter‐organ distribution of NDs intravenously injected to mice. In 2.5 h after the injection of NDs, the nanoparticles mainly accumulated in the lungs and liver of the animals – about 25 and 20%, respectively, of the initially injected NDs. The amounts of NDs accumulated in the heart and kidneys were considerably lower. Also, EPR spectrometry did not detect NDs in the blood, spleen, brain, and femoral muscles of mice. Ten days after injection, EPR spectrometry detected redistribution of NDs in mice. The amounts of nanoparticles decreased approximately by a factor of 3.5 in the lungs and increased almost by a factor of 3 in the liver; NDs were detected in the spleen. This study suggests ways to use EPR spectrometry to study the distribution, accumulation, and elimination of detonation NDs injected into laboratory animals.