Open AccessBiologically‐synthesised ZnO/CuO/Ag nanocomposite using propolis extract and coated on the gauze for wound healing applications
Author(s) -
Bayrami Mahdi,
Bayrami Abolfazl,
HabibiYangjeh Aziz,
Shafeeyan Mohammad Saleh,
Feizpoor Solmaz,
Arvanagh Farid Mohammadi,
Nourani Mohammad Reza,
Taheri Ramezan Ali
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2020.0024
Subject(s) - thermogravimetric analysis , trichrome , wound healing , masson's trichrome stain , nanocomposite , materials science , fourier transform infrared spectroscopy , nuclear chemistry , scanning electron microscope , dermis , biomedical engineering , nanoparticle , haematoxylin , eosin , h&e stain , nanotechnology , chemistry , staining , chemical engineering , medicine , composite material , pathology , surgery , organic chemistry , engineering
Wound healing has long been recognised as a major clinical challenge for which stablishing more effective wound therapies is necessary. The generation of metallic nanocomposites using biological compounds is emerging as a new promising strategy for this purpose. In this study, four metallic nanoparticles (NPs) with propolis extract (Ext) and one without propolis including ZnO/Ext, ZnO/Ag/Ext, ZnO/CuO/Ext, ZnO/Ag/CuO/Ext and ZnO/W were prepared by microwave method and assessed for their wound healing activity on excision experimental model of wounds in rats. The developed nanocomposites have been characterised by physico‐chemical methods such as X‐ray diffraction, scanning electron microscopy, diffuse reflectance UV–vis spectroscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis and Brunauer–Emmett–Teller analyses. The wounded animals treated with the NPs/Ext in five groups for 18 days. Every 6 days, for measuring wound closure rate, three samples of each group were examined for histopathological analysis. The prepared tissue sections were investigated by haematoxylin and Eosin stainings for the formation of epidermis, dermis and muscular and Masson's trichrome staining for the formation of collagen fibres. These findings toughly support the probability of using this new ZnO/Ag/Ext materials dressing for a wound care performance with significant effect compared to other NPs.