Open AccessAmino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid
Author(s) -
Saikia Kongkona,
Senthil Kumar Ponnusamy,
Karanam Rathankumar Abiram,
SaiLavanyaa Sundar,
Srinivasan Lakshmi,
Subramanian Sivanesan,
Cabana Hubert,
Gosselin Mathilde,
Vinoth Kumar Vaidyanathan
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2020.0021
Subject(s) - candida antarctica , chemistry , glutaraldehyde , lipase , nuclear chemistry , mesoporous silica , hydrogen peroxide , organic chemistry , alkyl , turnover number , chromatography , catalysis , mesoporous material , enzyme
In the present study, amino‐functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5‐furandicarboxylic acid (FDCA) from 2,5‐diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77–79% FDCA in 48 h at 30°C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol −1 and 129.59 h −1 , respectively, for ethyl acetate, against 6297.65 mol mol −1 and 131.2 h −1 , respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production.