Open AccessFerulic acid‐loaded collagen hydrolysate and polycaprolactone nanofibres for tissue engineering applications
Author(s) -
Kumar Chinnaiyan Senthil,
Soloman Agnes Mary,
Thangam Ramar,
Perumal Ramesh Kannan,
Gopinath Arun,
Madhan Balaraman
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2019.0281
Subject(s) - polycaprolactone , response surface methodology , electrospinning , materials science , box–behnken design , tissue engineering , composite number , ferulic acid , hydrolysate , central composite design , biomedical engineering , polyester , composite material , chromatography , chemistry , polymer , organic chemistry , hydrolysis , medicine
There is a great need for the progress of composite biomaterials, which are effective for tissue engineering applications. In this work, the development of composite electrospun nanofibres based on polycaprolactone (PCL) and collagen hydrolysate (CH) loaded with ferulic acid (FA) for the treatment of chronic wounds. Response Surface Methodology (RSM) has been applied to nanofibres factor manufacturing assisted by electrospinning. For wound healing applications, the authors have created the efficacy of CH, and PCL membranes can act as a stable, protective cover for wound, enabling continuous FA release. The findings of the RSM showed a reasonably good fit with a polynomial equation of the second order which was statistically acceptable at P < 0.05. The optimised parameters include the quantity of hydrolysate collagen, the voltage applied and the distance from tip‐to‐collector. Based on the Box–Behnken design, the RSM was used to create a mathematical model and optimise nanofibres with minimum diameter production conditions. Using FTIR, TGA and SEM, optimised nanofibres were defined. In vitro, cytocompatibility trials showed that there was an important cytocompatibility of the optimised nanofibres, which was proved by cell proliferation and cell morphology. In this research, the mixed nanofibres of PCL and CH with ferulic could be a potential biomaterial for wound healing.