Open AccessHigh‐efficiency Ni 2+ ‐NTA/PAA magnetic beads with specific separation on His‐tagged protein
Author(s) -
Wang Wenjing,
Zhou Fengzhen,
Cheng Xiyao,
Su Zhengding,
Guo Huiling
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2019.0271
Subject(s) - china , christian ministry , people's republic , pharmaceutics , political science , chinese academy of sciences , the republic , library science , engineering , microbiology and biotechnology , medicine , biology , law , computer science , pharmacology , theology , philosophy
To effective capture and universal enrichment of His‐tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe 3 O 4 nanoparticles, connect NTA, and Ni 2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His‐tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe 3 O 4 /MPS@PAA/NTA‐Ni 2+ was 93.4 mg/g. Compared with High‐Affinity Ni‐NTA Resin and Ni‐NTA Magnetic Agarose Beads, Fe 3 O 4 /MPS@PAA/NTA‐Ni 2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His‐tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.