Open AccessFacile nano‐free electrochemiluminescence biosensor for detection of sulphamethoxazole via tris(2,2′‐bipyridyl)ruthenium(II) and N ‐methyl pyrrolidone recognition
Author(s) -
Tong Xiyuan,
Jin Shiyao,
Zhao Yingdai,
Gai Yue,
E Yifeng,
Li Dan
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2019.0257
Subject(s) - electrochemiluminescence , ruthenium , detection limit , luminescence , analyte , aqueous solution , chemistry , quenching (fluorescence) , biosensor , analytical chemistry (journal) , chemiluminescence , excited state , nanomaterials , photochemistry , fluorescence , materials science , chromatography , nanotechnology , organic chemistry , optoelectronics , catalysis , physics , quantum mechanics , biochemistry , nuclear physics
The electrochemiluminescence (ECL) system based on the ruthenium complex has become a powerful tool in the field of analytical chemistry. However, the non‐aqueous ECL luminescence system, which does not involve complex nano‐modification, has not been widely used for the determination of analytes. In this study, N ‐methyl pyrrolidone was selected as the solvent, and it could also act as a co‐reactant of Rubpy3 2 + . Based on this, a simple ECL system without nanomaterials was established. Strong ECL was generated. Furthermore, a quenching effect between the excited state of Rubpy3 2 +and sulphamethoxazole (SMZ) was observed. Based on this, a sensitive ECL sensor for detecting SMZ is constructed. A linear relationship between ECL signal quenching intensity (Δ I ) and the logarithm of SMZ concentration (log C ) in the concentration range of 1 × 10 −7 –1 × 10 −5 mol/l is obtained. The limit of detection is as low as 3.33 × 10 −9 mol/l. The method has been applied to the detection of SMZ in tap water samples with different concentration levels with satisfactory results, and the recovery was 95.3–102.6%.