Open AccessStudies on antimicrobial and wound healing applications of gauze coated with CHX–Ag hybrid NPs
Author(s) -
Viswanathan Kaliyaperumal,
Bharathi Babu Diviya,
Karuppannan Chitra,
Sanjeevi Thiyagarajan,
Nithiyanantham Madan,
Arul Kumar Kandasamy,
Murugaiyan Latha Mala Priyadhershini,
Gopal Dhinakarraj,
Muthusamy Raman
Publication year - 2020
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2019.0187
Subject(s) - antimicrobial , biofilm , staphylococcus aureus , wound healing , chemistry , in vivo , silver nanoparticle , silver nitrate , microbiology and biotechnology , chlorhexidine , nuclear chemistry , antibiotics , nanoparticle , bacteria , materials science , nanotechnology , dentistry , medicine , surgery , biochemistry , organic chemistry , biology , genetics
In this study, chlorhexidine (CHX)–silver (Ag) hybrid nanoparticles (NPs) coated gauze was developed, and their bactericidal effect and in vivo wound healing capacities were tested. A new method was developed to synthesise the NPs, wherein Ag nitrate mixed with sodium (Na) metaphosphate and reduced using Na borohydride. Finally, CHX digluconate was added to form the hybrid NPs. To study the antibacterial efficacy of particles, the minimal inhibition concentration and biofilm degradation capacity against Gram‐positive and Gram‐negative bacteria was studied using Escherichia coli and Staphylococcus aureus . The results indicated that the NP inhibited biofilm formation and was bactericidal as well. The gauze was doped with NPs, and its wound healing property was evaluated using mice model. Results indicated that the wound healing process was fastened by using the NPs gauze doped with NPs without the administration of antibiotics.