Open AccessNucleic acid detection strategy using gold nanoprobe of two diverse origin
Author(s) -
Vinayagam Saranya,
Rajaiah Paramasivan,
Mukerjee Amitava,
Natarajan Chandrasekaran
Publication year - 2019
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2018.5332
Subject(s) - nanoprobe , colloidal gold , detection limit , nucleic acid , nanotechnology , biomolecule , naked eye , dna , nanoparticle , fluorescence , chemistry , biophysics , materials science , chromatography , biology , biochemistry , physics , quantum mechanics
In recent years, nanoparticles especially with gold and silver nanoparticles based point of care diagnostic methods is being developed for the lethal diseases like dengue. This study focused to work on the dengue virus detection in a simplest method using gold nanoparticles probe (AuNPs) with thiol tagged single strand DNA (ss‐DNA). A sensitive, fluorescence‐based detection strategy was designed to examine and quantified the hybridisation process and also elucidated the behaviour of AuNPs before and after interaction of biomolecule. The detection process was focused on aggregation of gold nanoprobe in the presence of complementary strand (target region). Hence the percentage of aggregation was measured and as a result, the limit of detection was found to be 10 −6 dilutions. Current detection method was highly sensitive, easy to perform and the reaction timing is rapid between 5 and 10 min, and it can be observed through naked eye.