Open AccessIn vitro biological evaluation of anti‐diabetic activity of organic–inorganic hybrid gold nanoparticles
Author(s) -
Uma Suganya Kuttalam Sambamoorthy,
Govindaraju Kasivelu,
Veena Vani Chitoor,
Premanathan Mariappan,
Ganesh Kumar Vijaya Kumar
Publication year - 2019
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2018.5139
Subject(s) - wortmannin , colloidal gold , chemistry , glucose uptake , in vitro , insulin , propanoic acid , biochemistry , pharmacology , endocrinology , biology , nanoparticle , pi3k/akt/mtor pathway , materials science , apoptosis , nanotechnology
Diabetes mellitus has been considered as a heterogeneous metabolic disorder characterised by complete or relative impairment in the production of insulin by pancreatic β‐cells or insulin resistance. In the present study, propanoic acid, an active biocomponent isolated from Cassia auriculata is employed for the synthesis of propanoic acid functionalised gold nanoparticles (Pa@AuNPs) and its anti‐diabetic activity has been demonstrated in vitro. In vitro cytotoxicity of synthesised Pa@AuNPs was performed in L6 myotubes. The mode of action of Pa@AuNPs exhibiting anti‐diabetic potential was validated by glucose uptake assay in the presence of Genistein (insulin receptor tyrosine kinase inhibitor) and Wortmannin (Phosphatidyl inositide kinase inhibitor). Pa@AuNPs exhibited significant glucose uptake in L6 myotubes with maximum uptake at 50 ng/ml. Assays were performed to study the potential of Pa@AuNPs in the inhibition of protein‐tyrosine phosphatase 1B, α‐glucosidases, and α‐amylase activity.