Open AccessEvaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract
Author(s) -
Gholami Maryam,
Shahzamani Kiana,
Marzban Abdolrazagh,
Lashgarian Hamed Esmaeil
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2018.5110
Subject(s) - silver nanoparticle , fourier transform infrared spectroscopy , nuclear chemistry , scanning electron microscope , chemistry , aqueous solution , nanoparticle , antibacterial activity , spectroscopy , absorption spectroscopy , absorption (acoustics) , biomolecule , materials science , nanotechnology , bacteria , chemical engineering , organic chemistry , biology , physics , quantum mechanics , engineering , composite material , genetics
Development of a green chemistry process for the synthesis of silver nanoparticles (AgNPs) has become a focus of interest. Characteristics of AgNPs were determined using techniques, such as ultraviolet–visible spectroscopy (UV–vis), Fourier transform infrared (FTIR) analysis, scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy and X‐ray diffraction (XRD). The synthesised AgNPs using Thymus kotschyanus had the most growth inhibition against gram‐positive bacteria such as Staphylococcus aureus and Bacillus subtilise , while the growth inhibition of AgNPs at 1000–500 µg/ml occurred against Klebsiella pneumonia and at 1000–250 µg/ml of AgNPs was observed against E. coli . The UV–vis absorption spectra confirmed the formation of the AgNPs with the characteristic peak at 415 nm and SEM micrograph acknowledged spherical particles in a nanosize range. FTIR measured the possible biomolecules that are responsible for stabilisation of AgNPs. XRD analysis exhibited the crystalline nature of AgNPs and showed face‐centred cubic structure. The synthesised AgNPs revealed significant antibacterial activity against gram‐positive bacteria.