Open AccessGreen synthesis of silver nanoparticle by cauliflower extract: characterisation and antibacterial activity against storage
Author(s) -
Oda Amjed Mirza,
Abdulkadhim Hussein,
Jabuk Sura I.A.,
Hashim Rahma,
Fadhil Iman,
Alaa Dhay,
Kareem Ali
Publication year - 2019
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2018.5095
Subject(s) - silver nanoparticle , fourier transform infrared spectroscopy , silver nitrate , nuclear chemistry , scanning electron microscope , surface plasmon resonance , chemistry , antibacterial activity , particle size , adsorption , nanoparticle , stabiliser , analytical chemistry (journal) , materials science , nanotechnology , chemical engineering , chromatography , bacteria , organic chemistry , genetics , biology , engineering , composite material
Green synthesis of silver nanoparticles (AgNPs) was accomplished using different volumes of cauliflower extract and 0.001 M silver nitrate solution at 80°C for 15 min. A brownish‐red solution of AgNPs formed was tested by ultraviolet–visible absorption spectroscopy, Fourier‐transform infrared (FTIR), scanning electron microscopy (SEM), and X‐ray diffraction (XRD). Surface plasmon resonance of AgNPs appeared at 416 nm. Also, the kinetic of AgNPs formation was studied and follows a sigmoidal pattern. Storing time was studied for the freshly prepared AgNPs after 60 days. FTIR analysis shows the adsorption of active components on AgNPs surface, and these components are responsible for reduction besides working as a stabiliser like a capping agent, also FTIR analysis of AgNPs after storage showed no change in peaks location. The SEM exhibited a globular shape of AgNPs, and the particle size ranged from 25 to 100 nm, while the XRD particle size calculation was 25 nm with cubic phase lattice. The antibacterial activity was tested against Gram‐positive and ‐negative bacteria showed an inhibition zone of 16–27 mm and the antibacterial activity tested for the same bacteria after storage for about 10 months showed an inhibition zone of 6–10 mm.