Open AccessFSE–Ag complex NS: preparation and evaluation of antibacterial activity
Author(s) -
Kalhapure Rahul S.,
Bolla Pradeep,
Dominguez Delfina C.,
Dahal Amit,
Boddu Sai H.S.,
Renukuntla Jwala
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2017.0284
Subject(s) - zeta potential , antibacterial activity , solubility , nuclear chemistry , broth microdilution , minimum inhibitory concentration , sonication , particle size , dispersity , dynamic light scattering , antimicrobial , chemistry , escherichia coli , staphylococcus aureus , chromatography , scanning electron microscope , materials science , nanotechnology , nanoparticle , bacteria , polymer chemistry , organic chemistry , biochemistry , biology , gene , composite material , genetics
Silver (Ag) complexes of drugs and their nanosystems have great potential as antibacterials. Recently, an Ag complex of furosemide (Ag–FSE) has shown to be a promising antimicrobial. However, poor solubility of Ag–FSE could hamper its introduction into clinics. Therefore, the authors developed a nanosuspension of Ag–FSE (Ag–FSE_NS) for its solubility and antibacterial activity enhancement. The aim of this study was to introduce a novel nanoantibiotic with enhanced antibacterial efficacy. Ag–FSE_NS was prepared by precipitation–ultrasonication technique. Size, polydispersity index (PI) and zeta potential (ZP) of prepared Ag–FSE_NS were measured by dynamic light scattering, whereas surface morphology was determined using scanning electron microscopy (SEM). In vitro antibacterial activity was evaluated against Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa using broth microdilution method. Size, PI and ZP of optimised Ag–FSE_NS1 were 191.2 ± 19.34 nm, 0.465 ± 0.059 and −55.7 ± 8.18 mV, respectively. SEM revealed that Ag–FSE_NS1 particles were rod or needle‐like with smooth surfaces. Saturation solubility of Ag–FSE in NS increased eight‐fold than pure Ag–FSE. Ag–FSE_NS1 exhibited two‐fold and eight‐fold enhancements in activity against E. coli and S. aureus , respectively. The results obtained showed that developed Ag–FSE_NS1 holds a promise as a topical antibacterial.