Open AccessPreparation of alginate oligosaccharide nanoliposomes and an analysis of their inhibitory effects on Caco‐2 cells
Author(s) -
Zhang Zhuanzhuan,
Dai Xianjun
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2017.0260
Subject(s) - liposome , caco 2 , chemistry , phosphatidylcholine , particle size , oligosaccharide , nanoparticle , biophysics , chromatography , cell , biochemistry , nanotechnology , membrane , materials science , phospholipid , biology
The conditions were optimised for preparing Alginate oligosaccharide (AOS) nanoliposomes, and Caco‐2 cell experiments were carried out to examine their antitumour effects. The optimal formulation of AOS nanoliposomes was as follows: a phosphatidylcholine‐to‐cholesterol ratio of 5.12, AOS concentration of 8.44 mg/mL, Tween 80 concentration of 1.11%, and organic phase to aqueous phase ratio of 5.25. Under the above conditions, the experimental encapsulation efficiency was 65.84%, and the AOS nanoliposomes exhibited a small particle size of 323 nm. After Caco‐2 cells were treated with AOS liposomes and AOS for 24 h, AOS nanoliposomes inhibited the growth of Caco‐2 cells to a greater extent than AOS at concentrations of 0.0625, 0.125, 0.25, 0.5 and 1 mg/mL ( P < 0.01). LDH leakage exhibited a concentration‐dependent increase following treatment with 0.5‐1 mg/mL AOS nanoliposomes, and the inhibitory effect of AOS nanoliposomes exhibited a more significant difference than AOS ( P < 0.01). Cells treated with 0.5 mg/mL and 1 mg/mL AOS nanoliposomes displayed a substantial and significant increase in activity compared with AOS ( P < 0.01). Based on these results, AOS nanoliposomes exerted a more significant effect on inhibiting Caco‐2 cell proliferation than AOS.