Open AccessPlasmonic photothermal therapy of colon cancer cells utilising gold nanoshells: an in vitro study
Author(s) -
Koohi Samira Rasouli,
Derakhshan Mohammad Ali,
Faridani Faramarz,
Muhammad Nejad Samad,
Amanpour Saeid,
Tajerian Roksana,
Yarmahmoodi Masood,
FaridiMajidi Reza
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2017.0144
Subject(s) - nanoshell , fourier transform infrared spectroscopy , photothermal therapy , materials science , scanning electron microscope , transmission electron microscopy , plasmon , absorbance , nanoparticle , colloidal gold , spectroscopy , dynamic light scattering , surface plasmon resonance , nanotechnology , nuclear chemistry , analytical chemistry (journal) , chemistry , chemical engineering , optoelectronics , chromatography , physics , quantum mechanics , engineering , composite material
In this study, gold nanoshell (GNS) were synthesised utilising the Halas method. The obtained nanoparticles (NPs) were characterised by Fourier‐transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy and dynamic light scattering. FTIR spectra demonstrated the successful functionalisation of silica NP with 3‐aminopropyl trimethoxysilane. SEM and TEM images showed the morphology and diameter of the synthesised silica NPs (137 ± 26 nm) and GNS. UV–Vis spectrum illustrated the maximum absorbance of the resultant GNS and their average hydrodynamic diameter was 159 nm. For in vitro study, HCT‐116 cells were exposed to gold nanoshells and intense pulsed light in different experiment groups. The results showed that exposing the cells to nanoshells and 30 s irradiation would efficiently decrease the viability percentage of the cells to about 30% compared with the control. A continued exposure of 4 min decreased the viability of the cancer cells to 20%. The results demonstrated that photothermal therapy would be promising in treatment of colon cancer cells utilising gold nanoshells.