Open AccessIn vitro antibacterial activity of ceftazidime, unlike ciprofloxacin, improves in the presence of ZnO nanofluids under acidic conditions
Author(s) -
Javedani Bafekr Jalil,
Jalal Razieh
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2017.0119
Subject(s) - acinetobacter baumannii , enterococcus faecalis , ceftazidime , ciprofloxacin , antibiotics , antimicrobial , antibacterial activity , chemistry , microbiology and biotechnology , nuclear chemistry , bacteria , biochemistry , pseudomonas aeruginosa , biology , organic chemistry , escherichia coli , genetics , gene
The development of antibiotic resistance among hospital pathogens has provided a great need for new antimicrobial agents. Zinc oxide nanoparticles (ZnO NPs) in combination with various antibiotics can act as a reducing agent for antibiotic resistance. The aim of this study was to investigate the influence and the mechanism of ZnO NPs on the antimicrobial activity of ciprofloxacin (CP) and ceftazidime (CAZ) against Enterococcus faecalis ( E. faecalis ) and Acinetobacter baumannii ( A. baumannii ) bacteria in acidic conditions (pH 5.5). ZnO NPs were synthesised using the solvothermal method and characterised. The MIC90 value of ZnO NPs against A. baumannii was 0.25 mg ml −1 and its highest growth‐inhibitory activity was observed at 0.125 mg ml −1 for E. faecalis. The Fourier transform infrared spectroscopy spectra of ZnO NPs treated with antibiotics showed the interaction between ZnO NPs and each of the two antibiotics. ZnO NPs at a sub‐inhibitory concentration had no effect on the antibacterial activity of CP and CAZ against E. faecalis and CP against A. baumannii. The action mechanism of ZnO NPs for enhancing the antibacterial efficacy of CAZ against A. baumannii was evaluated. ZnO NPs caused to increase in the antibacterial activity of CAZ against A. baumannii , possibly through the release of Zn 2+ and increasing of membrane permeability.