Open AccessGreen synthesis, characterisation and biological studies of AgNPs prepared using Shivlingi ( Bryonia laciniosa ) seed extract
Author(s) -
Balkrishna Acharya,
Sharma Niti,
Sharma Vinay Kumar,
Mishra Nayan Deep,
Joshi Chandra Shekhar
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2017.0099
Subject(s) - nanoparticle , fourier transform infrared spectroscopy , transmission electron microscopy , nuclear chemistry , dynamic light scattering , raman spectroscopy , silver nanoparticle , scanning electron microscope , absorption (acoustics) , chemistry , absorption spectroscopy , cytotoxicity , materials science , nanotechnology , chemical engineering , biochemistry , in vitro , physics , optics , quantum mechanics , engineering , composite material
Green synthesis of silver nanoparticles (AgNPs) using Shivlingi ( Bryonia laciniosa ) seed extract was carried out. Characterisation of synthesised nanoparticles was accomplished through the optical absorption and photoluminescence spectrum, X‐ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The XRD analysis further confirmed the size of nanoparticles ∼15 nm. TEM images revealed homogeneous spherical ∼10 nm Bryonia extract capped AgNPs. The biological studies indicated that both Bryonia seed extract and the nanoparticles lack anti‐microbial activity; however, the nanoparticles had better cytotoxicity and total antioxidant activity. The Lethal concentration (LC) 50 value of water extract and the nanoparticles were found to be 1091 and 592 μg/ml, respectively. The lower LC 50 of nanoparticles indicates that it is more cytotoxic than the crude extract. The results indicate that the Bryonia seed is safe to be used as a medicine and the formation of their nanoparticle has further enriched the chemical reactivity, energy absorption and biological mobility.