Open AccessBiofilm reduction, cell proliferation, anthelmintic and cytotoxicity effect of green synthesised silver nanoparticle using Artemisia vulgaris extract
Author(s) -
Ejaz Kiran,
Sadia Haleema,
Zia Ghazna,
Nazir Shabnam,
Raza Abida,
Ali Shaukat,
Iqbal Tariq,
Andleeb Saiqa
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2017.0096
Subject(s) - silver nanoparticle , cytotoxicity , biofilm , viability assay , chemistry , antibacterial activity , nuclear chemistry , mtt assay , hela , nanoparticle , microbiology and biotechnology , traditional medicine , bacteria , biology , nanotechnology , in vitro , materials science , biochemistry , medicine , genetics
Infectious diseases are caused by etiological agents. Nanotechnology has been used to minimise the effect of clinical pathogens which have resistance to antibiotics. In current research synthesis, characterisation and biological activities of green synthesised nanoparticles using Artemisia vulgaris extract have been done. The characterisation of AgNPs was carried out using Fourier transform infrared spectroscopy, UV‐Vis spectrophotometry, and scanning electron microscopy. Anti‐biofilm, cell viability, antibacterial, brine shrimp lethality, and deoxyribonucleic acid protection effects have been screened. UV‐Vis spectra showed the absorption peak of synthesised nanoparticles at 400 nm. FT‐IR indicated the involvement of the functional group in the preparation of AgNPs. SEM showed the spherical shape of AgNPs with 30 nm diameter. Biological screening results revealed the antibacterial effect against clinical bacterial pathogens. Biofilm reduction and cell viability assay also supported the antibacterial effect. Cytotoxicity effect was recorded as 100% at 200 μg/ml through brine shrimp lethality assay. Protein kinase inhibition zones recorded for AgNPs (16 mm bald) compared with A. vulgaris extract (11 mm bald). It has been concluded that green synthesised AgNPs are more effective against infectious pathogens and could be used as a potential source for therapeutic drugs.