Open AccessApoptotic efficacy and antiproliferative potential of silver nanoparticles synthesised from aqueous extract of sumac ( Rhus coriaria L.)
Author(s) -
Ghorbani Parisa,
Namvar Farideh,
HomayouniTabrizi Masoud,
Soltani Mozhgan,
Karimi Ehsan,
Yaghmaei Parichehreh
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2017.0080
Subject(s) - propidium iodide , apoptosis , dapi , acridine orange , cytotoxic t cell , mtt assay , trypan blue , mcf 7 , chemistry , silver nanoparticle , cytotoxicity , cancer cell , microbiology and biotechnology , biochemistry , traditional medicine , pharmacology , biology , in vitro , programmed cell death , cancer , nanotechnology , medicine , nanoparticle , materials science , human breast , genetics
Currently, nanotechnology and nanoparticles (NPs) are recognised due to their extensive applications in medicine and the treatment of certain diseases, including cancer. Silver NPs (AgNPs) synthesised by environmentally friendly method exhibit a high medical potential. This study was conducted to determine the cytotoxic and apoptotic effects of AgNPs synthesised from sumac (Anacardiaceae family) fruit aqueous extract (AgSu/NPs) on human breast cancer cells (MCF‐7). The anti‐proliferative effect of AgSu/NPs was determined by MTT assay. The apoptotic properties of AgSu/NPs were assessed by morphological analysis and acridine orange/propidium iodide (AO/PI) and DAPI staining. The mechanism of apoptosis induction in treated cells was investigated using molecular analysis. Overall results of morphological examination and cytotoxic assay revealed that AgSu/NPs exert a concentration‐dependent inhibitory effect on the viability of MCF‐7 cells (IC50 of ∼10 µmol/48 h). AO/PI staining confirmed the occurrence of apoptosis in cells treated with AgSu/NPs. In addition, molecular analysis demonstrated that the apoptosis in MCF‐7 cells exposed to AgSu/NPs was induced via up‐regulation of Bax and down‐regulation of Bcl‐2. These findings suggested the potential use of AgSu/NP as cytotoxic and pro‐apoptotic efficacy and its possible application in modern medicine for treating certain disorders, such as cancer.