Open AccessSynthesis, stability, and in vitro oral cancer cell toxicity of human serum albumin stabilised gold nanoflowers
Author(s) -
Uppal Abha,
Bose Biplab
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2017.0002
Subject(s) - in vitro , toxicity , human serum albumin , bovine serum albumin , cancer , albumin , chemistry , serum albumin , cancer research , materials science , nanotechnology , medicine , biochemistry , organic chemistry
Gold nanoflowers (GNFs) prepared by reduction of HAuCl 4 by ascorbic acid were capped with human serum albumin (HSA) by either electrostatic or covalent attachment to prevent their self‐aggregation. Measurement of surface plasmon resonance absorbance changes under different stress conditions showed that GNFs stabilised by covalent attachment of HSA were more stable than those stabilised by electrostatic attachment. Cytotoxicity of the covalently conjugated GNF was also studied in cultured human oral cancer cell lines by measuring the metabolic activity via 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay.