Open AccessFactors affecting the antibacterial activity of chitosan‐silver nanocomposite
Author(s) -
Sherif Hadeer H.A.,
Khalil Safaa K.H.,
Hegazi Ahmed G.,
Khalil Wafaa A.,
Moharram Mohamed A.
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2016.0249
Subject(s) - chitosan , silver nitrate , antibacterial activity , silver nanoparticle , nuclear chemistry , fourier transform infrared spectroscopy , nanocomposite , composite number , materials science , transmission electron microscopy , staphylococcus aureus , bacterial growth , antibacterial agent , nanoparticle , chemistry , bacteria , nanotechnology , chemical engineering , composite material , antibiotics , organic chemistry , biology , biochemistry , genetics , engineering
This study provides the optimum preparation parameters of chitosan‐silver nanoparticles composite (CSNC) with promising antibacterial activity against the most common bacterial infections found on burn wounds. CSNC was synthesised by simple green chemical reduction method with different preparation factors. Chitosan was used to reduce silver nitrate and stabilise silver nanoparticles in the medium. For this reason, spectroscopic and microscopic techniques as, ultraviolet‐visible Fourier transform infrared spectroscopy and transmission electron microscopy were used in the study of the molecular and morphological properties of the resultant composites. Furthermore, the composite was assessed in terms of Ag‐ions release by AAS and its efficacy as antibacterial material. As a result, CSNC showed stronger antibacterial effect than its individual components (chitosan and silver nitrate solutions) towards Gram‐positive (Staphylococcus aureus) and Gram‐negative (Pseudomonas aeruginosa and Escherichia coli) bacteria. CSNC prepared in this study showed highest inhibition percentage of bacterial growth up to 96% at concentration of 220 μg/ml.