Open AccessSensitive determination of uric acid by using graphene quantum dots as a new substrate for immobilisation of uric oxidase
Author(s) -
Yu HongWei,
Zhang Ze,
Shen Tao,
Jiang JingHui,
Chang Dong,
Pan HongZhi
Publication year - 2018
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2016.0221
Subject(s) - biosensor , urate oxidase , graphene , detection limit , substrate (aquarium) , dielectric spectroscopy , cyclic voltammetry , electrode , chemistry , materials science , quantum dot , analytical chemistry (journal) , electrochemistry , uric acid , nanotechnology , nuclear chemistry , chromatography , biochemistry , oceanography , geology
A novel strategy for highly sensitive electrochemical detection of uric acid (UA) was proposed based on graphene quantum dots (GQDs), GQDs were introduced as a suitable substrate for enzyme immobilisation. Uric oxidase (UOx) was immobilised on GQDs modified glassy carbon electrode (GCE). Transmission electron microscope, scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy techniques were used for characterising the electrochemical biosensor. The developed biosensor responds efficiently to UA presence over the concentration linear range 1–800 μM with the detection limit 0.3 μM. This novel biosensing platform based on UOx/GQDs electrode responded even more sensitively than that based on GCE modified by UOx alone. The inexpensive, reliable and sensitive sensing platform based on UOx/GQDs electrode provides wide potential applications in clinical.