Open AccessComparison effects of titanium dioxide nanoparticles on immune cells in adaptive and innate immune system
Author(s) -
Sukwong Patinya,
Kongseng Supunsa,
Chaicherd Sunisa,
Yoovathaworn Krongtong,
Tubtimkuna Suchakree,
Pissuwan Dakrong
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2016.0205
Subject(s) - jurkat cells , immune system , cytotoxic t cell , innate immune system , tumor necrosis factor alpha , viability assay , cytotoxicity , chemistry , titanium dioxide , microbiology and biotechnology , cell , materials science , immunology , biology , t cell , biochemistry , in vitro , metallurgy
Titanium dioxide nanoparticles (TiO 2 ‐NPs) have been increasingly mixed in food and daily use products. Therefore, the investigation of cytotoxic effects of TiO 2 ‐NPs is required to allay concerns of health effects related to contact with products containing TiO 2 ‐NPs. In this study, the authors demonstrated how TiO 2 ‐NPs impact on two main sub‐types of immune cells that play a major role in adaptive and innate immune system. Human T‐lymphocytes (Jurkat cells) and murine macrophages (RAW 264.7 cells) were used in this study. The authors results showed that cell viability of Jurkat and RAW 264.7 cells were significantly decreased, when cells were treated with TiO 2 ‐NPs at 250 and 500 µg/ml. However, the decrease of cell viability of RAW 264.7 cells was higher than that of Jurkat cells. A similar trend was also found in DNA fragmentation. An induction of reactive oxygen species was detected in both cells treated with TiO 2 ‐NPs at concentrations ≥25 µg/ml. A significant induction of tumour necrosis factor alpha (TNF‐α) was found in Jurkat and RAW 264.7 cells treated with 25 µg/ml TiO 2 ‐NPs. In contrast, there was no significant induction of interleukin‐6 (IL‐6) in both cells that were treated with different concentrations of TiO 2 ‐NPs.