Open AccessBacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics
Author(s) -
Ding Zhenhao,
Kasahara Hiromichi,
Nakano Michihiko,
Suehiro Junya
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2016.0186
Subject(s) - dielectrophoresis , microbead (research) , amplicon , bacteria , polymerase chain reaction , dna , electrode , chain reaction , electrophoresis , detection limit , chemistry , materials science , nanotechnology , chromatography , microbiology and biotechnology , biology , biochemistry , gene , genetics , photochemistry
In this study, an electrical DNA detection method was applied to bacterial detection. DNA was extracted from bacteria and amplified by polymerase chain reaction. The microbeads were labelled with amplicons, altering their surface conductance and therefore their dielectrophoresis characteristics. Amplicon‐labelled microbeads could thus be trapped within a high‐strength electric field, where they formed a pearl chain between the electrodes, resulting in an increased conductance between the electrodes. This method reduces the amplicon detection time from 1–2 h to 15 min, compared with the conventional method. The presented method realised quantitative detection of specific bacteria at concentrations above 1 × 10 5 and 2.4 × 10 4 CFU/ml for bacterial solutions with and without other bacterial presence, respectively.