Open AccessBiomimetic synthesis and anticancer activity of Eurycoma longifolia branch extract‐mediated silver nanoparticles
Author(s) -
Nallappan Devi,
Tollamadugu Prasad N.V.K.V,
Fauzi Agustine Nengsih,
Yaacob Nik Soriani,
Pasupuleti Visweswara Rao
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2016.0181
Subject(s) - silver nanoparticle , nuclear chemistry , chemistry , fourier transform infrared spectroscopy , silver nitrate , antimicrobial , nanoparticle , antioxidant , amide , attenuated total reflection , antibacterial activity , biochemistry , nanotechnology , organic chemistry , infrared spectroscopy , bacteria , materials science , biology , chemical engineering , genetics , engineering
In the present study, silver nanoparticles (AgNPs) were synthesised by adding 1 mM Ag nitrate solution to different concentrations (1%, 2.5%, 5%) of branch extracts of Eurycoma longifolia, a well known medicinal plant in South–East Asian countries. Characterisation of AgNPs was carried out using techniques such as ultraviolet–visible spectrophotometry, X‐ray diffractrometry, Fourier transform infrared–attenuated total reflection spectroscopy (FTIR–ATR), scanning electron microscopy. XRD analysis revealed face centre cubic structure of AgNPs and FTIR–ATR showed that primary and secondary amide groups in combination with the protein molecules present in the branch extract were responsible for the reduction and stabilisation of AgNPs. Furthermore, antioxidant [2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐Azino‐bis(3‐ethylbenzthiazoline‐6‐sulphonic acid)], antimicrobial and anticancer activities of AgNPs were investigated. The highest bactericidal activity of these biogenic AgNPs was found against Escherichia coli with zone inhibition of 11 mm. AgNPs exhibited significant anticancer activity against human glioma cells (DBTRG and U87) and human breast adenocarcinoma cells (MCF‐7 and MDA‐MB‐231) with IC 50 values of 33, 42, 60 and 38 µg/ml.