Open AccessAntioxidant, antibacterial and anticancer properties of phyto ‐ synthesised Artemisia quttensis Podlech extract mediated AgNPs
Author(s) -
Ghanbar Farinaz,
Mirzaie Amir,
Ashrafi Fatemeh,
Noorbazargan Hassan,
Dalirsaber Jalali Mojgan,
Salehi Soheil,
Sadat Shandiz Seyed Ataollah
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2016.0101
Subject(s) - propidium iodide , silver nanoparticle , chemistry , antibacterial activity , nuclear chemistry , cytotoxicity , zeta potential , antioxidant , mtt assay , nanotechnology , biochemistry , nanoparticle , apoptosis , bacteria , materials science , biology , in vitro , programmed cell death , genetics
The focus of this study is on a rapid and cost‐effective approach for the synthesis of silver nanoparticles (AgNPs) using Artemisia quttensis Podlech aerial parts extract and assessment of their antioxidant, antibacterial and anticancer activities. The prepared AgNPs were determined by ultraviolet–visible spectroscopy, X‐ray diffraction, Fourier transform infra‐red spectroscopy, transmission electron microscopy, scanning electron microscopy, energy‐dispersive spectroscopy, and dynamic light scattering and zeta‐potential analysis. The AgNPs and A. quttensis extract were evaluated for their antiradical scavenging activity by 2, 2‐diphenyl, 1‐picryl hydrazyl assay and anticancer activity against colon cancer (human colorectal adenocarcinoma cell line 29) compared with normal human embryonic kidney (HEK293) cells. Also, the prepared AgNPs were studied for its antibacterial activity. The AgNPs revealed a higher antioxidant activity compared with A. quttensis extract alone. The phyto‐synthesised AgNPs and A. quttensis extract showed a dose–response cytotoxicity effect against HT29 and HEK293 cells. As evidenced by Annexin V/propidium iodide staining, the number of apoptotic HT29 cells was significantly enhanced, following treatment with AgNPs as compared with untreated cells. Besides, the antibacterial property of the AgNPs indicated a significant effect against the selected pathogenic bacteria. These present obtained results show the potential applications of phyto‐synthesised AgNPs using A. quttensis aerial parts extract.