Open AccessEnhanced cytotoxic activity of AgNPs on retinoblastoma Y79 cell lines synthesised using marine seaweed Turbinaria ornata
Author(s) -
Remya Rajan Renuka,
Radhika Rajasree Santha Ravindranath,
Aranganathan Lakshminarayanan,
Suman Thodhal Yoganandham,
Gayathri Sathyamoorthy
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2016.0042
Subject(s) - chemistry , nuclear chemistry , zeta potential , silver nanoparticle , retinoblastoma , cytotoxicity , phototoxicity , fourier transform infrared spectroscopy , nanoparticle , nanotechnology , materials science , biochemistry , in vitro , chemical engineering , engineering , gene
Retinoblastoma is the most common intraocular malignancy basically occurs among children below five. Certain ocular treatments such as surgery, radiation therapy and chemotherapy are more likely to cause side effects. Here, a rapid method of synthesising silver nanoparticles (AgNPs) from the brown seaweed Turbinaria ornata and its cytotoxic efficacy against the retinoblastoma Y79 cell lines was studied. The AgNPs synthesis was determined by Ultraviolet–visible spectroscopy and was further characterised by X‐ray diffraction, High‐resolution transmission electron microscopy, zeta potential , Energy‐dispersive X‐ray spectroscopy, thermogravimetric analysis, Fourier transform infrared spectrum and inductively coupled plasma‐mass spectroscopy techniques. The synthesised AgNPs were found to be very stable and finely dispersed. The total phenolic content of the synthesised AgNPs was estimated at 43±2.52 mg/g gallic acid equivalent and the nanoparticles exhibited good scavenging activity analysed by 2, 2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulphonic acid) assay. Moreover, cytotoxicity of synthesised AgNPs against in vitro retinoblastoma Y79 cell lines showed a dose‐dependent response with an inhibitory concentration (IC 50 ) of 10.5 µg/mL. These results suggest that AgNPs could be a promising anticancer agent with enhanced activity in ocular treatment.