Open AccessCharacterisation of sol–gel method synthesised MgZnFe 2 O 4 nanoparticles and its cytotoxic effects on breast cancer cell line, MDA MB‐231 in vitro
Author(s) -
Nordin Noraini,
Kanagesan Samikannu,
Zamberi Nur Rizi,
Yeap Swee Keong,
Abu Nadiah,
Tamilselvan Subramani,
Hashim Mansor,
Alitheen Noorjahan Banu
Publication year - 2017
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2016.0007
Subject(s) - thermogravimetric analysis , nuclear chemistry , nanoparticle , differential thermal analysis , fourier transform infrared spectroscopy , cytotoxicity , calcination , particle size , materials science , mcf 7 , analytical chemistry (journal) , transmission electron microscopy , mtt assay , chemistry , chromatography , nanotechnology , in vitro , cancer cell , human breast , chemical engineering , biochemistry , organic chemistry , diffraction , medicine , cancer , engineering , optics , catalysis , physics
In this study, nanocrystalline magnesium zinc ferrite nanoparticles were successfully prepared by a simple sol–gel method using copper nitrate and ferric nitrate as raw materials. The calcined samples were characterised by differential thermal analysis/thermogravimetric analysis, Fourier transform infrared spectroscopy and X‐ray diffraction. Transmission electron microscopy revealed that the average particle size of the calcined sample was in a range of 17–41 nm with an average of 29 nm and has spherical size. A cytotoxicity test was performed on human breast cancer cells (MDA MB‐231) and (MCF‐7) at various concentrations starting from (0 µg/ml) to (800 µg/ml). The sample possessed a mild toxic effect toward MDA MB‐231 and MCF‐7 after being examined with MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyltetrazolium bromide) assay for up to 72 h of incubation. Higher reduction of cells viability was observed as the concentration of sample was increased in MDA MB‐231 cell line than in MCF‐7. Therefore, further cytotoxicity tests were performed on MDA MB‐231 cell line.