Open AccessLarvicidal activity of green synthesized silver nanoparticles using Excoecaria agallocha L. (Euphorbiaceae) leaf extract against Aedes aegypti
Author(s) -
Anil Kumar Vundru,
Ammani Kandru,
Jobina Rajkumari,
Parasuraman Paramanandham,
Siddhardha Busi
Publication year - 2016
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2015.0101
Subject(s) - aedes aegypti , instar , silver nanoparticle , nuclear chemistry , chloroform , aqueous solution , traditional medicine , larva , chemistry , solvent , botany , biology , chromatography , nanoparticle , organic chemistry , materials science , nanotechnology , medicine
Green synthesis of silver nanoparticles (AgNPs) using plant extracts has been achieved by eco‐friendly reducing and capping agents. The present study was conducted to evaluate the larvicidal efficacies of AgNPs synthesized using aqueous leaf extracts of Excoecaria agallocha against dengue vector, Aedes aegypti . The 3 rd and 4 th instar larvae of A. aegypti were exposed to various concentrations of aqueous extracts of E. agallocha , synthesized AgNPs and also crude solvent extracts (methanol and chloroform) for 24 h. The formation of AgNPs using aqueous leaf extracts was observed after 30 min with a characteristic colour change. The results recorded from UV‐Vis spectrum, XRD, FTIR, EDX, SEM and HR‐TEM were used to characterize and confirm the biosynthesis of AgNPs. The highest larvicidal efficacy of synthesized AgNPs was observed against 3 rd instar larvae at LC 50 4.65 mg/L, LC 90 14.17 mg/L and 4 th instar larvae with a concentration of LC 50 6.10 mg/L, LC 90 15.64 mg/L. A significant larvicidal activity was also observed with crude methanolic extracts against 3 rd instar larvae at a concentration LC 50 41.74 mg/L, LC 90 123.61 mg/L and 4 th instar larvae at a concentration of LC 50 52.06 mg/L, LC 90 166.40 mg/L as compared to the chloroform extract.