Open AccessShort interfering RNA therapeutics: nanocarriers, prospects and limitations
Author(s) -
Mehrotra Neha,
Tripathi Ravi Mani
Publication year - 2015
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2015.0018
Subject(s) - nanocarriers , small interfering rna , rna interference , rna , nanomedicine , gene silencing , endosome , nanotechnology , drug delivery , computational biology , biology , cell , nanoparticle , gene , materials science , genetics
Since the first experiment depicting gene inhibition using RNA interference mechanism, extensive research has been carried out to design targeted delivery systems that use short interfering RNAs (siRNAs) for gene expression regulation. Although several siRNAs loaded nanoparticle systems have reached clinical trial stage, cellular uptake, reticuloendothelial entrapment and endosomal escape still limit the efficacy of these drugs considerably. This review discusses about the RNA interference mechanism, nanostructures being used as non‐viral vectors for targeted delivery, limitations of the common delivery systems and the current siRNA‐loaded nanoparticle formulations undergoing clinical testing.