Open AccessComparative analysis of biosynthesised and chemosynthesised silver nanoparticles with special reference to their antibacterial activity against pathogens
Author(s) -
Bawskar Manisha,
Deshmukh Shivaji,
Bansod Sunita,
Gade Aniket,
Rai Mahendra
Publication year - 2015
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2014.0032
Subject(s) - silver nanoparticle , antibacterial activity , nanoparticle , zeta potential , nuclear chemistry , chemistry , reducing agent , fourier transform infrared spectroscopy , nanotechnology , materials science , chemical engineering , bacteria , organic chemistry , biology , engineering , genetics
The present study reports the synthesis of silver nanoparticles (AgNPs) using both biological and chemical routes to find out the best method for control of their size and activity. The fungal agent ( Fusarium oxysporum) and the plant ( Azadirachta indica) were found to be the best source for AgNPs synthesis. Both biosynthesis and chemosynthesis were achieved by challenging filtrate with AgNO 3 (1 mM) solution. The synthesised nanoparticles were characterised by ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis (LM20), zeta potential measurement and transmission electron microscopy. The biologically synthesised nanoparticles were spherical, polydispersed and in the range of 10–40 nm, while chemically synthesised nanoparticles were highly monodispersed with a size of 5 nm. The antimicrobial assay against Escherichia coli and Staphylococcus aureus proved biogenic AgNPs to be more potent antibacterial agents than chemically synthesised AgNPs. The possible antibacterial mechanism of AgNPs has also been discussed. Biogenic AgNPs have shown more activity because of the protein capping and their mode of entry into the bacterial cell. These findings may encourage the use of biosynthesis over the chemosynthesis method.