Open AccessComponent analyses of urinary nanocrystallites of uric acid stone formers by combination of high‐resolution transmission electron microscopy, fast Fourier transformation, energy dispersive X‐ray spectroscopy, X‐ray diffraction and Fourier transform infrared spectroscopy
Author(s) -
Sun XinYuan,
Xue JunFa,
Xia ZhiYue,
Ouyang JianMing
Publication year - 2015
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2014.0017
Subject(s) - high resolution transmission electron microscopy , fourier transform infrared spectroscopy , transmission electron microscopy , spectroscopy , analytical chemistry (journal) , materials science , oxalate , fourier transform spectroscopy , chemistry , optics , physics , inorganic chemistry , nanotechnology , chromatography , quantum mechanics
This study aimed to analyse the components of nanocrystallites in urines of patients with uric acid (UA) stones. X‐ray diffraction (XRD), Fourier transform infrared spectroscopy, high‐resolution transmission electron microscopy (HRTEM), fast Fourier transformation (FFT) of HRTEM, and energy dispersive X‐ray spectroscopy (EDS) were performed to analyse the components of these nanocrystallites. XRD and FFT showed that the main component of urinary nanocrystallites was UA, which contains a small amount of calcium oxalate monohydrate and phosphates. EDS showed the characteristic absorption peaks of C, O, Ca and P. The formation of UA stones was closely related to a large number of UA nanocrystallites in urine. A combination of HRTEM, FFT, EDS and XRD analyses could be performed accurately to analyse the components of urinary nanocrystallites.