Open AccessSensitive electrochemical biosensing of H 2 O 2 based on cobalt nanoparticles synthesised in iron storage protein molecules, ferritin
Author(s) -
Rafipour Ronak,
Kashanian Soheila,
Abasi Tarighat Fereshteh
Publication year - 2014
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2013.0050
Subject(s) - ferritin , cobalt , nanoparticle , biosensor , electrochemistry , nanobiotechnology , molecule , chemistry , nanotechnology , materials science , nuclear chemistry , inorganic chemistry , biochemistry , electrode , organic chemistry
In this report, a highly sensitive electrochemical biosensor based on cobaltferritin immobilised on a self‐assembled monolayer modied gold electrode for determination of hydrogen peroxide (H 2 O 2 ) in phosphate buffer solution (pH 7.5) was investigated. The modied electrode showed excellent electrochemical activity for oxidation of H 2 O 2 . The response to H 2 O 2 on the modied electrode was examined using linear sweep and differential pulse voltammetries. In phosphate buffer (pH 7.5, 0.1 M), the fabricated biosensor exhibited a linear dependence ( R = 0.989) on the concentration of H 2 O 2 from 2.49 × 10 −9 to 1.91 × 10 −8 M, a high sensitivity of −0.4099 µA/nM and detection limit of 2.48 × 10 −9 based on a signal‐to‐noise ratio of 3. Charge transfer coefcient ( α ) and the exchange current ( i 0 ) of oxidation for H 2 O 2 were found to be 0.57 and 7.55 A, respectively. It has been shown that, this modied electrode is able to determine H 2 O 2 with a high sensitivity, low detection limit and high selectivity.