Open AccessEffect of gold nanoparticles on photodynamic efficiency of 5‐aminolevolenic acid photosensitiser in epidermal carcinoma cell line: an in vitro study
Author(s) -
Amini Seyed Mohammad,
Kharrazi Sharmin,
Hadizadeh Mahnaz,
Fateh Mohsen,
Saber Reza
Publication year - 2013
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2013.0021
Subject(s) - photodynamic therapy , cytotoxicity , colloidal gold , bromide , mtt assay , nanoparticle , chemistry , in vitro , nanotechnology , nuclear chemistry , cell culture , combinatorial chemistry , materials science , biophysics , biochemistry , organic chemistry , biology , genetics
In the recent years, enhanced functionality of treatment systems based on nanostructures has attracted a lot of interest. Photodynamic therapy (PDT) is one such treatment method. Here, the authors report the results of the investigations on synthesis and characterisation of gold nanoparticles (GNPs) and their application in PDT along with 5‐aminolevolenic acid (5‐ALA) (as photosensitiser) with no conjugation. Three sizes of GNPs were synthesised and their cytotoxicity was investigated by using MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay on epidermal carcinoma cell line. The results showed that the PDT efficiency of ALA increased in presence of GNPs. This effect was more considerable for 4 nm particles.