Open AccessLawsonia inermis‐ mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel
Author(s) -
Gupta Arpita,
Bonde Shital R.,
Gaikwad Swapnil,
Ingle Avinash,
Gade Aniket K.,
Rai Mahendra
Publication year - 2014
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
ISSN - 1751-875X
DOI - 10.1049/iet-nbt.2013.0015
Subject(s) - lawsonia inermis , microsporum canis , nanogel , antimicrobial , nuclear chemistry , zeta potential , silver nanoparticle , fourier transform infrared spectroscopy , chemistry , candida albicans , nanoparticle , microbiology and biotechnology , materials science , nanotechnology , drug delivery , biology , organic chemistry , chemical engineering , antifungal , medicine , traditional medicine , engineering
Lawsonia inermis mediated synthesis of silver nanoparticles (Ag‐NPs) and its efficacy against Candida albicans , Microsporum canis , Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two‐step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag‐NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO 3 . The Ag‐NPs were characterized by Ultraviolet‐Visible (UV–Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag‐NPs. The antimicrobial activity of Ag‐NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag‐NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag‐NPs may be an answer to the drug‐resistant microorganisms.