Open AccessEvaluation of therapeutic potential of nanosilver particles synthesised using aloin in experimental murine mastitis model
Author(s) -
Chaitanya Kumar Thota Venkata,
Muralidhar Yegireddy,
Prasad Pagadala Eswara,
Prasad Tollamadugu Naga Venkata Krishna Vara,
Alpha Raj Mekapogu
Publication year - 2013
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/iet-nbt.2012.0045
Subject(s) - mastitis , chemistry , staphylococcus aureus , superoxide dismutase , thiobarbituric acid , catalase , intraperitoneal injection , microbiology and biotechnology , histopathology , inoculation , food science , antibiotics , distilled water , pharmacology , lipid peroxidation , biochemistry , antioxidant , bacteria , biology , immunology , pathology , medicine , chromatography , genetics
Nanobiotechnology is an emerging biological branch of nanotechnology. Application of nanoparticles with specific size and shape in biology has already shown unforeseen and interesting results. A study was conducted to evaluate the therapeutic potential of phytogenically derived aloin mediated nanosilver particles (AAgNPs), prepared by reduction of silver nitrate with aloin, in Staphylococcus aureus induced murine mastitis. A total of 40 female mice were divided into five groups of eight animals each. Group I served as lactating control, groups II‐V were inoculated with 20 μl of 24 h broth culture of S. aureus containing 4.0 × 105 cfu/quarter under ketamine anaesthesia. After 6 h post inoculation, groups III and IV received 20 μl of aloin nanosilver (AAgNPs) through intramammary and intraperitoneal routes, respectively. Group V received antibiotic cefepime at 1 mg/kg body weight through the intra‐peritoneal route. After 18 h post‐treatment, serum C reactive protein, weights of mammary glands, mammary gland bacterial load, thiobarbituric acid reactive substances content, reduced glutathione content, superoxide dismutase activity and catalase activity and histopathology were determined. The compound showed a minimum inhibitory concentration of 21.8 ng/ml against S. aureus . Significant reduction (98%) in poly‐morpho nuclear cell infiltration was observed with AAgNPs than antibiotic (50%).