Open AccessThermofluidic chip containing virtual thermal wells
Author(s) -
Ferguson Andrew J.,
Hayes Matthew J.,
Kirkpatrick Blair C.,
Lin Yenchun,
Narayan Vijay,
Prak Albert
Publication year - 2019
Publication title -
engineering biology
Language(s) - English
Resource type - Journals
ISSN - 2398-6182
DOI - 10.1049/enb.2018.5010
Subject(s) - microscale chemistry , chip , thermal , thermal management of electronic devices and systems , fluidics , phosphoramidite , materials science , nanotechnology , computer science , engineering , mechanical engineering , chemistry , electrical engineering , physics , dna , thermodynamics , telecommunications , biochemistry , mathematics education , mathematics , oligonucleotide
The authors describe a thermofluidic chip on which microscale islands of controlled temperature are formed within an open fluidic environment. The chip forms part of the authors’ technology for thermally controlled DNA synthesis, whereby the site‐specific temperature control enables site‐specific addressability of chemical reactions, for example, related to the phosphoramidite cycle. Here, the authors discuss the principle of the chip, supporting the thermal well concept by means of simulations as well as by showing a prototype thermal array device.