Open AccessProduction of recombinant collagen: state of the art and challenges
Author(s) -
Wang Tianyi,
Lew Jiewei,
Premkumar Jayaraman,
Poh Chueh Loo,
Win Naing May
Publication year - 2017
Publication title -
engineering biology
Language(s) - English
Resource type - Journals
ISSN - 2398-6182
DOI - 10.1049/enb.2017.0003
Subject(s) - recombinant dna , immunogenicity , biocompatibility , computational biology , nanotechnology , chemistry , biology , microbiology and biotechnology , biochemistry , materials science , immunology , gene , immune system , organic chemistry
Collagen, which is often used in healthcare materials and biomedical research, is largely extracted from animal sources. Recombinant human collagen has the potential to be a promising alternative to animal collagen which has many shortcomings, including immunogenicity and lack of biocompatibility. Currently, recombinant human collagen has been expressed in both eukaryotic and prokaryotic hosts with varying degrees of success. One issue with recombinant collagen across all hosts is the inability to achieve full length collagen with native amounts of post‐translational modifications, prompting much exciting research in this direction. There has also been much effort in improving yield and biomimicry of recombinant collagen. This review discusses collagen structure and current methods for extracting animal collagen, before introducing current research in synthesising recombinant human collagen in various hosts, and finally highlighting challenges in the field.