Open AccessPortable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay
Author(s) -
Liu Juntao,
Qu Shuxue,
Luo Jinping,
Wang Bin,
Cai Xinxia
Publication year - 2017
Publication title -
chinese journal of electronics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.267
H-Index - 25
eISSN - 2075-5597
pISSN - 1022-4653
DOI - 10.1049/cje.2016.10.010
Subject(s) - chemiluminescence , immunoassay , chromatography , carcinoembryonic antigen , chemiluminescent immunoassay , horseradish peroxidase , correlation coefficient , chemistry , analytical chemistry (journal) , computer science , antibody , medicine , cancer , immunology , enzyme , biochemistry , machine learning
Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemiluminescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno‐sandwich scheme as‐say with Horseradish peroxidase (HRP) labeled anti‐CEA antibody and Immunomagnetic beads (IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to 1000ng/mL, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of 0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.